Primary monolayer culture of rat ependymal cells: An ultrastructural study.
نویسندگان
چکیده
منابع مشابه
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
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Hepatic parenchymal cells from adult rats, established in vitro as a monolayer, have been evaluated by electron microscopy . Within 24 h after the initial seeding, the incubated cells were polygonal and in close apposition with three to six neighboring cells . The ultrastructure of the monolayer cells was examined at this time and after 3 and 10 days of incubation . With the exception of a few ...
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Hepatic parenchymal cells from adult rats, established in vitro as a monolayer, have been evaluated by electron microscopy. Within 24 h after the initial seeding, the incubated cells were polygonal and in close apposition with three to six neighboring cells. The ultrastructure of the monolayer cells was examined at this time and after 3 and 10 days of incubation. With the exception of a few enl...
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The aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in Sterlet, Acipenser ruthenus. Oocytes for the primary culture were obtained from the ovaries of a 6 years old Sterlet 729 g in weight and 47 cm in total length. The oocytes were in advanced vitellogenesis stage (PI >10). A part of the ovary (containing about 300 follicles) w...
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The morphogenetic potential of human colon cancer cells was examined by combining cells with foetal rat mesenchyme in organ culture. Out of four cell lines examined, LS174T cells formed glandular structures composed of a simple columnar or cuboidal epithelium with a lumen in the centre of the cell mass. Ultrastructurally, these gland-forming LS174T cells exhibited polarity, with nuclei located ...
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ژورنال
عنوان ژورنال: Archivum histologicum japonicum
سال: 1983
ISSN: 0004-0681
DOI: 10.1679/aohc.46.191